ABSTRACT: Speaking anxiety is the condition when the students feel worry, nervous, not confident to speak English in front of many people. Speaking anxiety can happen to any students because of the lack of English knowledge, communication apprehension, and afraid of making mistake. The purpose of this research is to find out anxiety factors of the most anxious students in English. This study use qualitative research that examines 6 students at second year English education study program in Muhammadiyah Kotabumi University. The instrument used interview and observation. The result of this study indicated that anxiety factors of the most anxious students in English consist of 1) fear of being in public and shyness, 2) fear of being negative evaluation, 3) fear of being speaking inaccurately, 4) poor of English proficiency, 5) unfamiliar task, 6) attitude to English, 7) and Background of English.